Biology : Lab Report

Surname 1NameCourseProfessorMarch 25, 2019Lab reportIntroductionExercise 3With the recent advancements in the field of cloning, the study of genes has advanced agreat deal. The three main advancements to which these developments can be attributed toinclude: the discovery of restriction enzymes, the ability to propagate individual genes on smallDNA molecules (plasmids) and the ability to selectively increase the amount of DNA coding in adesired gene with the polymerase chain reaction. restriction enzymes are responsible for thefragmentation of DNA at specific sites. The main motive behind cloning is to replicate a desiredgene independently of the original chromosome.Cloning begins when the required chromosome is cut using a specific restriction enzyme.The isolated DNA is then replicated in a vector, which is independent of the originalchromosome. A cloning vector is the DNA molecule that acts as a carrier for DNA fragmentsand it is responsible for their replication (Schildkraut, 2001). High copy number vectors arepreferred because they yield greater number of DNA. Enzyme ligase then links two pieces ofDNA together in order to form the final vector.Exercise 4: Clone verificationSurname 2After cloning a region of DNA, one needs to characterize this region (the insert).Restriction enzymes come in handy when confirming that the correct DNA has been replicated.This method has some disadvantages such as the overreliance of the process on the work of there ...


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